Inducción de la diferenciación en células eritroleucémicas humanas y murinas: Síntesis de espectrina

Abstract

The differentiation of human (K562) and murine (Friend) erythroleukemic cells was studied by stimulation with hemin and DMSO respectively. After the second day of stimulation, hemin iduces the production of hemoglobin up to 18% in K562 cells. In Friend cells, DMSO produces a clear increase in hemoglobin synthesis after the fifth day of stimulation when about 30% of the cells differentiate. Both cellular lines showed an increase in the synthesis of a protein with an electrophoretic mobility similar to the erythrocytic B-spectrin. The amount of this protein increased 5 times in the murine cells and 2.5 times in the human cells during induction. The synthesis of a protein with electrophoretic mobility similar to that of a-spectrin was increased in induce K562 cells. Nevertheless, none of these proteins reacted with a polyspecific rabbit antiserum against human B-spectrin.