Comparación entre métodos de cultivo independientes y dependientes para la detección de bacteriemia transitoria en individuos diabéticos con periodontitis crónica

  • Anna Carolina Ratto-Tespestini Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil
  • Paula Juliana Pérez-Chaparro Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil
  • Giuseppe Alexandre Romito Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil
  • Luciene Cristina Figueiredo Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil
  • Marcelo Faveri Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil
  • Hilana Paula Carillo Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil
  • Priscila Larcher Biological Medial Science Institute (ICB), University of São Paulo, São Paulo, Brazil
  • Magda Feres Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Palabras clave: bacteriemia, reacción en cadena de la polimerasa en tiempo real, periodontitis, diabetes mellitus/diagnóstico, microbiología

Resumen

Introducción. Las bacteriemias de origen oral pueden ocurrir después de procedimientos odontológicos y de otros actos cotidianos. Algunas condiciones de la cavidad oral favorecen las bacteriemias como en el caso de pacientes con diabetes mellitus y periodontitis que presentan inflamación gingival exacerbada.
Objetivo. Comparar la eficacia de un método independiente de cultivo (PCR cuantitativa) y otro dependiente (BacT-ALERT 3D®) en la detección de la bacteriemia.
Materiales y métodos. Se tomaron muestras de sangre de individuos con diabetes mellitus de tipo II y periodontitis, antes y después de la masticación de manzana. Una alícuota se procesó por el sistema automatizado de hemocultivo (BacT-ALERT 3D®) y se monitorizó durante 15 días; la otra alícuota fue tratada para la extracción del ADN y procesada por RT-PCR usando un conjunto de cebadores de 16S rDNA exclusivos para bacterias.
Resultados. En las muestras tomadas antes de masticar se confirmó la ausencia de bacterias mediante los dos métodos. En las muestras tomadas después de masticar la presencia de bacterias se evidenció únicamente en dos hemocultivos y en ninguna de las muestras se detectó la presencia de bacterias con el método de RT-PCR.
Conclusiones. La PCR cuantitativa no mostró mayor eficacia que el BacT-ALERT 3D® en la detección de la bacteriemia de origen oral.

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Publicado
2016-03-01
Cómo citar
Ratto-Tespestini, A., Pérez-Chaparro, P., Romito, G., Figueiredo, L., Faveri, M., Carillo, H., Larcher, P., & Feres, M. (2016). Comparación entre métodos de cultivo independientes y dependientes para la detección de bacteriemia transitoria en individuos diabéticos con periodontitis crónica. Biomédica, 36(1), 156-161. https://doi.org/10.7705/biomedica.v36i1.2674
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