DOI: https://doi.org/10.7705/biomedica.v37i0.3452

Señal filogenética de la región Cytb-SertRNA-IG1-ND1 en Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913

Andrés López-Rubio, Juan David Suaza, Charles Porter, Sandra Uribe, Gabriel Bedoya, Iván Darío Vélez

Resumen


Introducción. El ADN mitocondrial ha demostrado su utilidad para el estudio de la evolución en los insectos. Existen algunos genes mitocondriales como el citocromo b (Cytb) y el gen de transferencia para el aminoácido serina (SertRNA) que pueden usarse en el diagnóstico de especies estrechamente relacionadas.
Objetivo. Explorar la utilidad filogenética de la región Cytb-SertRNA-IG1-ND1 para detectar posibles especies crípticas en Anopheles neivai.
Materiales y métodos. Se recolectaron especímenes en Colombia, Guatemala y en la localidad tipo en Panamá, los cuales se secuenciaron y se compararon mediante el polimorfismo de ADN en toda la región y mediante la simulación de estructuras secundarias del gen SertRNA.
Resultados. Se obtuvieron las secuencias de especímenes de A. neivai (34) y A. pholidotus (2).
Conclusiones. Se detectaron algunos polimorfismos para la regiónCytb-SertRNA-IG1-ND1 en A. neivai, pero no así especies crípticas.


Palabras clave


Anopheles; malaria; ARN de transferencia; ADN mitocondrial; Colombia; Panamá; Guatemala

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Referencias


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