Modulación de la resistencia a norfloxacina de Staphylococcus aureus por Croton A. campestris y Ocimum gratissimum L.
Palabras clave:
Croton campestres A., Ocimum gratissimum L., modificación de la resistencia, antibióticos, Sistema de eflujo de NorA, Staphylococcus aureus.
Resumen
Introducción. Algunas especies de Staphylococcus suelen ser reconocidas como agentes etiológicos de muchas infecciones oportunistas en animales y em humanos. Este estudio es la primera prueba del
cambio en la resistencia de la actividad antibiótica por Croton campestris A. y Ocimum gratissimum L. contra cepas multirresistentes de Staphylococcus aureus.
Objetivo. Ensayar la actividad antibacteriana de los extractos hexánicos y metanólicos de Croton campestris A. y Ocimum gratissimum L. sola y en combinación con norfloxacina sobre la cepa SA1199B.
Materiales y métodos. Se analizó la concentración inhibitoria mínima (CIM) y el efecto modulador de los extractos usando el ensayo de microtitulación.
Resultados. Por el hecho de que la CIM observada no era clínicamente relevante (CIM: 512 ≥ 1.024 mg/ml), la actividad antibiótica de norfloxacina fue potenciada cuando se combinó este antibiótico con
concentraciones subinhibitorias de los extractos, principalmente con los extractos hexánicos.
Conclusiones. Estos resultados indican que los extractos ensayados presentan compuestos que pueden ser utilizados como inhibidores putativos de la bomba de eflujo, lo que indica que Croton campestris A. y Ocimum gratissimum L. pueden ser una fuente de productos derivados de plantas con actividad modificadora de antibióticos.
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Referencias bibliográficas
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13. Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Muigai AW, Mukiama TK. Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. Afr J Biotechnol. 2007;6:760-5.
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21. Gibbons S. Anti-staphylococcal plant natural products. Nat Prod Rep. 2004;21:263-77.
22. Coutinho HD, Costa JG, Lima EO, Siqueira-Júnior JP. Additive effects of Hyptis martiusii Benth with aminoglycosides against Escherichia coli. Indian J Med Res. 2010;131:106-8.
23. Rodrigues FF, Costa JG, Coutinho HD. Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri. Phytomedicine. 2009;16:1052-5.
24. Ferreira FS, Brito SV, Costa JG, Alves RR, Coutinho HD, Almeida WO. Is the body fat of the lizard Tupinambis merianae effective against bacterial infections? J Ethnopharmacol. 2009;126:233-7.
25. Nicolson K, Evans G, O´Toole PW. Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett. 1999;179:233-9.
26. Juven J, Kanner J, Schved F, Weisslowicz H. Factors that interact with antimicrobial action of thyme essential oil and its active constituents. J Appl Bacteriol. 1994;76:626-31.
27. Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 2004;94:223-53.
28. Helander IM, Alakomi HL, Latva-Kala K, Sandholm TM, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agricult Food Chem. 1998;46:3590-5.
29. Wendakoon C, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Protect. 1995;58:280-3.
30. Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97-110.
2. Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I. Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol Lett. 2004;230:191-5.
3. Verhoeff JD, Beaujean DH, Vlok HA, Baars AA, Meyler A, Werkwn VD. A dutch approach to methicillin-resistance Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1999;18:461-6.
4. Coutinho HD, Costa JG, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP. Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Mentha arvensis L. and chlorpromazine. Chemotherapy. 2008;54:328-30.
5. Coutinho HD, Costa JGM, Siqueira-Júnior JP, Lima EO. In vitro anti- staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus-MRSA strains. Braz J Pharmacogn. 2008;18(Suppl.):670-5.
6. Oliveira FQ, Gobira BC, Guimarães CJ, Batista JM, Barreto M, Souza M. Plants species indicated in odontology. Braz J Pharmacogn. 2007;17:466-76.
7. Saúde-Guimarães DA, Faria AR. Natural compounds with anti-Trypanossoma cruzi activity. Braz J Pharmacogn. 2007;17:455-65.
8. Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. Michiganensis. Crop Protect. 2003;22:39-44.
9. de Heluani CS, Catalán CA, Hernández LR, Burgueño-Tapia E, Joseph-Nathan P. Three new diterpenoids based on novel sarcopetalene skeleton from Croton sarcopetalus. J Nat Prod. 2000;63:222-5.
10. Cruz GL. Dicionário das plantas úteis do Brasil. 2ª ed. Rio de Janeiro: Ed. EDEL; 1982.
11. Lorenzi H, Matos FJ. Plantas medicinais do Brasil: nativas e exóticas. Nova Odessa: Instituto Plantarum de Estudos da Flora; 2002.
12. Martins JR, Alvarenga AA. Leaf Anatomy of alfavacacravo plants cultivated under colored nets. Ciênc Rural. 2008;39:82-7.
13. Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Muigai AW, Mukiama TK. Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. Afr J Biotechnol. 2007;6:760-5.
14. Ueda-Nakamura T, Mendonça-Filho RR, Morgado-Díaz JA, Korehisa MP, Prado DF, Cortez AGD, et al. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int. 2006;55:99-105.
15. Kaatz GW, Seo SM, Ruble CA. Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1993;37:1086-94.
16. Kaatz GW, Seo SM. Inducible NorA-mediated multidrug resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1995;39:2650-5.
17. Matos FJ. Introdução à Fitoquímica Experimental. 2ª Ed. Fortaleza: Edições UFC; 1997.
18. Javadpour MM, Juban MM, Lo WC, Bishop SM, Alberty JB, Cowell SM, et al. De novo antimicrobial peptides with low mammalian cell toxicity. J Med Chem. 1996;39:3107-13.
19. Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from natural sources. J Antimicrob Chemother. 2007;59:1247-60.
20. Senatore F, Rigano D, Formisano C, Grassia A, Basile A, Sorbo S. Phytogrowth-inhibitory and antibacterial activity of Verbascum sinuatum. Fitoterapia 2007;78:244-7.
21. Gibbons S. Anti-staphylococcal plant natural products. Nat Prod Rep. 2004;21:263-77.
22. Coutinho HD, Costa JG, Lima EO, Siqueira-Júnior JP. Additive effects of Hyptis martiusii Benth with aminoglycosides against Escherichia coli. Indian J Med Res. 2010;131:106-8.
23. Rodrigues FF, Costa JG, Coutinho HD. Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri. Phytomedicine. 2009;16:1052-5.
24. Ferreira FS, Brito SV, Costa JG, Alves RR, Coutinho HD, Almeida WO. Is the body fat of the lizard Tupinambis merianae effective against bacterial infections? J Ethnopharmacol. 2009;126:233-7.
25. Nicolson K, Evans G, O´Toole PW. Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett. 1999;179:233-9.
26. Juven J, Kanner J, Schved F, Weisslowicz H. Factors that interact with antimicrobial action of thyme essential oil and its active constituents. J Appl Bacteriol. 1994;76:626-31.
27. Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 2004;94:223-53.
28. Helander IM, Alakomi HL, Latva-Kala K, Sandholm TM, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agricult Food Chem. 1998;46:3590-5.
29. Wendakoon C, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Protect. 1995;58:280-3.
30. Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97-110.
Cómo citar
1.
Coutinho HD, Matias EF, Santos KK, Santos FA, Morais-Braga MFB, Souza TM, et al. Modulación de la resistencia a norfloxacina de Staphylococcus aureus por Croton A. campestris y Ocimum gratissimum L. biomedica [Internet]. 30 de junio de 2011 [citado 19 de abril de 2024];31(4):608-12. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/409
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